What is the recommended screening and treatment for hepatitis?

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Last updated: October 23, 2025View editorial policy

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Recommended Hepatitis Screening and Treatment Guidelines

Universal screening for hepatitis B and C is recommended for all adults aged 18 years and older at least once in their lifetime, with additional risk-based testing for those with ongoing risk factors. 1, 2

Hepatitis B Screening

Who to Screen

  • All adults aged ≥18 years should be screened at least once in their lifetime 3
  • Persons from countries with HBsAg prevalence ≥2% (Asia, Africa, Pacific Islands, parts of South America, Eastern Europe) 3
  • Persons with high-risk behaviors (injection drug use, men who have sex with men) 3
  • HIV-positive individuals 3
  • Household contacts or sexual partners of HBV-infected persons 3
  • Patients who will undergo immunosuppressive therapy 1

Recommended Testing Panel

  • Initial screening should include three tests: 4
    • Hepatitis B surface antigen (HBsAg)
    • Hepatitis B core antibody (anti-HBc) total or IgG
    • Antibody to hepatitis B surface antigen (anti-HBs)

Interpretation of Hepatitis B Results

Test Results Interpretation
HBsAg+, anti-HBc+, anti-HBs- Active HBV infection (chronic) [4]
HBsAg-, anti-HBc+, anti-HBs+ Resolved HBV infection [4]
HBsAg-, anti-HBc+, anti-HBs- Isolated core antibody (possible occult HBV) [4]
HBsAg-, anti-HBc-, anti-HBs+ Immunity from vaccination [4]
HBsAg-, anti-HBc-, anti-HBs- Susceptible to HBV infection [4]

Additional Testing for Positive HBsAg

  • For positive HBsAg results, order: 4
    • HBeAg
    • Anti-HBe
    • Quantitative HBV DNA

Hepatitis C Screening

Who to Screen

  • Universal one-time screening for all adults aged 18-79 years 2, 5
  • All pregnant women during each pregnancy 5
  • Risk-based screening for persons <18 years with risk factors 2
  • Periodic testing for those with ongoing risk factors 2
  • Annual testing for people who inject drugs and men with HIV who have unprotected sex with men 2

Recommended Testing Approach

  • Initial screening with HCV-antibody testing with reflex HCV RNA PCR testing 2
    • Two-step process: HCV-antibody testing followed by reflex HCV RNA PCR testing for positive results 2
    • Reflex testing requires only a single blood collection, eliminating need for return visit 2

Special Testing Considerations

  • For recent exposure (within 6 months), consider HCV RNA testing or follow-up antibody testing ≥6 months after exposure 2
  • For immunocompromised patients, consider direct HCV RNA testing 2
  • For patients at risk of reinfection, use HCV RNA testing since antibody tests remain positive after prior clearance 2

Treatment Recommendations

Hepatitis B Treatment

  • All HBsAg-positive persons should be referred to a specialist or primary care provider experienced in treating hepatitis B 1
  • Treatment decisions should be based on: 1
    • HBeAg status
    • ALT levels
    • HBV DNA levels
    • Evidence of liver inflammation/fibrosis
  • First-line treatment options include pegylated interferon alfa-2a, entecavir, and tenofovir 6

Hepatitis C Treatment

  • For treatment-naive adults without cirrhosis or with compensated cirrhosis: 5
    • Eight weeks of glecaprevir/pibrentasvir, OR
    • Twelve weeks of sofosbuvir/velpatasvir
  • These regimens result in >95% cure rates 5
  • Sustained virologic response (undetectable HCV RNA 12 weeks after completing therapy) is considered a virologic cure 5

Post-Diagnosis Management

Hepatitis B

  • Initial evaluation should include: 1
    • Thorough history and physical examination
    • Family history of HBV infection and liver cancer
    • Assessment of risk factors for coinfections
    • Alcohol use evaluation
  • Laboratory tests should assess: 1
    • Liver disease severity
    • Markers of HBV replication
    • Testing for coinfection with other viruses in at-risk individuals
  • Screening for hepatocellular carcinoma should be conducted in all HBsAg-positive persons ≥20 years old 1

Hepatitis C

  • For patients with compensated cirrhosis: 5
    • Surveillance for hepatocellular carcinoma with abdominal ultrasonography every six months
    • Upper endoscopy every two to three years for esophageal varices
  • In the absence of cirrhosis, no liver-related follow-up is recommended 5

Common Pitfalls to Avoid

  • Do not order IgM anti-HBc for routine screening; only order when acute hepatitis B is suspected 4
  • Do not rely solely on HBsAg for comprehensive HBV screening 4
  • Do not assume vaccination history eliminates the need for testing 4
  • Do not interpret isolated anti-HBc as definitively indicating occult infection without further testing 4
  • Relying solely on HCV antibody testing can miss active infection or incorrectly classify resolved infections as current 2

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Hepatitis C Virus Infection Screening Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Hepatitis B Virus Screening Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Hepatitis B Testing and Interpretation

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

Hepatitis C: Diagnosis and Management.

American family physician, 2021

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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