How should epinephrine (adrenaline) injections be stored?

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Storage of Epinephrine (Adrenaline) Injections

Store epinephrine injections at controlled room temperature (20°C to 25°C or 68°F to 77°F), protected from light and freezing, and inspect the solution before each use—discard if discolored, cloudy, or contains particles. 1

Temperature Requirements

  • Controlled room temperature of 20°C to 25°C (68°F to 77°F) is the FDA-mandated storage range, with permitted excursions between 15°C to 30°C. 1

  • Epinephrine is light-sensitive and must be protected from both light exposure and freezing temperatures. 1

  • Refrigeration (2°C to 8°C) may actually reduce degradation compared to room temperature storage, though this is not required by FDA labeling. 2

Critical Storage Pitfalls to Avoid

  • Never store epinephrine at excessively high temperatures (above 30°C), as constant heat exposure causes significant degradation—storage at 70°C for 10 days reduces epinephrine content to only 77.7% of labeled dose. 3

  • Avoid storing in vehicles or locations with extreme temperature fluctuations, particularly ambulances or cars in hot climates, as temperatures can reach 34°C or higher. 4

  • Do not use epinephrine that has been stored at excessively high temperatures while still hot, and even after cooling, such devices cannot be relied upon to deliver the labeled dose. 3

Visual Inspection Before Use

  • Epinephrine should be clear and colorless—any discoloration (yellow, brown, or pink) indicates oxidation and degradation. 5

  • Discard the solution immediately if it is colored, cloudy, or contains particulate matter. 1

  • The presence of crystals in the solution also indicates contamination and requires discarding the medication. 5

  • Inspect all ampules and vials visually before each use, regardless of the expiration date, as degradation can occur without visible discoloration or precipitation. 6

Expiration and Replacement

  • Check autoinjector expiration dates regularly and renew prescriptions promptly before expiration. 6

  • For multi-dose vials, discard the vial and contents 30 days after initial use. 1

  • In situations of medication scarcity, expired epinephrine can be maintained as an emergency reserve if stored properly, though this should not be routine practice. 5

Real-World Temperature Considerations

  • Research shows that brief temperature excursions in real-world conditions may be less detrimental than previously suggested—freezing and limited heat excursions did not result in significant epinephrine degradation in most studies. 2

  • Epinephrine stored at average temperatures of 27°C to 34°C in drug storage rooms and ambulances in tropical climates remained stable with 90% to 115% of drug content remaining after 4 weeks. 4

  • However, constant exposure to extreme heat (70°C) is far more damaging than cyclic temperature fluctuations, with constant heat causing more rapid degradation. 2, 3

Storage in Prefilled Syringes

  • Epinephrine at 1 mg/mL concentration stored in prefilled syringes remains stable and sterile for at least 90 days when properly stored. 7

  • No bacterial or fungal growth was detected in prefilled syringes after 90 days of storage. 7

  • This option may be considered when autoinjectors are not available or affordable, though autoinjectors remain the preferred method of administration. 7

References

Research

A systematic review of epinephrine degradation with exposure to excessive heat or cold.

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, 2016

Research

Stability of adrenaline in ambulance and drug storage room Narenthorn Center, Rajavithi Hospital.

Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 2013

Guideline

Anaphylaxis Management with Epinephrine

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

A systematic review of epinephrine stability and sterility with storage in a syringe.

Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology, 2019

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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