Which skeletal sites should be included in bone density testing, and must the lumbar spine always be measured?

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Last updated: February 11, 2026View editorial policy

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Bone Density Measurement Sites: Standard Protocol

Bone mineral density measurement should be performed at the lumbar spine (L1-L4), total hip, and femoral neck in all patients, with the one-third radius added only when indicated by specific clinical circumstances. 1

Core Skeletal Sites for Standard DXA Protocol

The 2025 European guidelines establish a clear hierarchy for bone density assessment:

  • Lumbar spine (L1-L4) is a mandatory measurement site and should be included in every standard DXA examination alongside hip measurements. 1, 2

  • Both total hip and femoral neck must be measured as part of the standard protocol, as these sites provide complementary diagnostic information. 1

  • The one-third radius is measured only when indicated, specifically when hip or spine measurements cannot be accurately obtained due to artifacts, hardware, or severe degenerative changes. 1

Why the Spine Cannot Be Omitted

The evidence strongly supports mandatory spine inclusion:

  • Spine-only osteoporosis is common and clinically significant. Research demonstrates that relying solely on hip measurements fails to identify 13-16% of patients with osteoporosis who have low bone density exclusively at the spine. 3, 4

  • Differential bone loss occurs at different skeletal sites. The spine and hip lose bone at different rates, making single-site measurement inadequate for comprehensive fracture risk assessment. 3

  • Vertebral fracture risk is missed without spine measurement. The majority of patients with normal hip BMD but low spine BMD are at significantly increased risk for vertebral fractures. 3

  • Diagnostic classification requires the lowest T-score from any measured site. The WHO operational definition of osteoporosis (T-score ≤ -2.5) applies to the lumbar spine, femoral neck, total hip, or one-third radius, with diagnosis based on the lowest value. 1

When Vertebral Levels Can Be Excluded

While the spine must be measured, specific vertebral bodies may be omitted under limited circumstances:

  • Up to 2 vertebral levels from L1-L4 can be excluded when structural artifacts are present, such as compression fractures, severe facet joint osteoarthritis, or spondylosis. 2

  • The same vertebral levels must be used for all follow-up scans to ensure valid longitudinal comparison. 2

Bilateral Hip Measurement Consideration

Recent evidence suggests potential benefit of measuring both hips:

  • Measuring both hips identifies an additional 13-16% of osteoporotic patients compared to single hip measurement, with only 55 seconds of additional scan time. 4

  • Current guidelines recommend measuring either hip, though bilateral measurement may be considered when resources permit. 1

Common Clinical Pitfalls

  • Do not rely on hip measurement alone, as this misses a substantial proportion of patients with spine-predominant osteoporosis who require treatment. 3

  • Do not use Ward's area for diagnosis, as this region should not be included in diagnostic assessment. 5

  • Do not use lateral spine BMD for osteoporosis diagnosis. 5

  • Forearm measurement is reserved for specific situations where central sites cannot be accurately measured, not as a routine screening site. 1

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

BMD Measurement Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Research

A prospective study of discordance in diagnosis of osteoporosis using spine and proximal femur bone densitometry.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, 2003

Research

Dual Hip DXA. Is it Time to Change Standard Protocol?

Journal of clinical densitometry : the official journal of the International Society for Clinical Densitometry, 2022

Research

Which central dual X-ray absorptiometry skeletal sites and regions of interest should be used to determine the diagnosis of osteoporosis?

Journal of clinical densitometry : the official journal of the International Society for Clinical Densitometry, 2002

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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