What is the recommended quadruple therapy regimen for H pylori (Helicobacter pylori) infection?

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H. pylori Quadruple Therapy

Bismuth quadruple therapy is the preferred first-line treatment for H. pylori infection, consisting of a PPI (twice daily), bismuth subcitrate (120-140 mg 3-4 times daily), metronidazole (500 mg 3-4 times daily), and tetracycline (500 mg 4 times daily) for 14 days. 1, 2

Why Bismuth Quadruple Therapy is Preferred

  • All major guidelines (American College of Gastroenterology, Toronto Consensus, and Maastricht V/Florence Consensus) recommend bismuth quadruple therapy as first-line treatment, particularly in areas with high dual resistance to clarithromycin and metronidazole. 1

  • Bismuth quadruple therapy is favored because bacterial resistance to bismuth is extremely rare, and it maintains acceptable success rates even in strains displaying in vitro metronidazole resistance. 1, 3

  • This regimen avoids clarithromycin, which has seen global resistance rates increase from 9% in 1998 to 17.6% in 2008-2009, making traditional triple therapy increasingly ineffective. 2, 4

Specific Dosing Regimen

The standard bismuth quadruple therapy includes: 3

  • PPI (high-dose): Esomeprazole 20 mg or rabeprazole 20 mg twice daily (avoid pantoprazole due to lower potency)
  • Bismuth subcitrate: 120-140 mg 3-4 times daily
  • Tetracycline hydrochloride: 500 mg 4 times daily
  • Metronidazole: 500 mg 3-4 times daily
  • Duration: 14 days (not 10 days, despite some guidelines listing 10-14 days as acceptable)

Critical Optimization Factors

High-dose PPI (twice daily) is essential and increases eradication efficacy by 6-10% compared to standard doses by reducing gastric acidity and enhancing antibiotic activity. 2, 4 Take PPIs 30 minutes before eating on an empty stomach without concomitant antacids. 1

The 14-day duration is superior to shorter regimens, improving eradication success by approximately 5% compared to 7-10 day courses. 2, 4 The Toronto Consensus specifically recommends 14 days for all first-line treatments to maximize first-attempt success. 1

Alternative First-Line Option: Concomitant (Non-Bismuth) Quadruple Therapy

If bismuth is unavailable, concomitant therapy is an acceptable alternative, consisting of: 1, 2

  • PPI twice daily
  • Amoxicillin 1000 mg twice daily
  • Metronidazole 500 mg twice daily
  • Clarithromycin 500 mg twice daily
  • Duration: 14 days

This regimen is appropriate for patients from areas of high clarithromycin resistance where bismuth is not available. 1

When NOT to Use Bismuth Quadruple Therapy

Do not use standard triple therapy (PPI + clarithromycin + amoxicillin) in areas where clarithromycin resistance exceeds 15-20%, as eradication rates become unacceptably low. 2, 4, 3 This restriction applies to most regions globally given current resistance patterns.

Second-Line Treatment After Quadruple Therapy Failure

If bismuth quadruple therapy fails, use levofloxacin-based triple therapy: 1, 2, 4

  • PPI twice daily
  • Amoxicillin 1000 mg twice daily
  • Levofloxacin 500 mg once daily (or 250 mg twice daily)
  • Duration: 14 days

After two failed eradication attempts with confirmed patient adherence, obtain H. pylori susceptibility testing to guide subsequent regimens. 1, 2

Common Pitfalls to Avoid

  • Never use doxycycline instead of tetracycline in bismuth quadruple therapy, as multiple studies demonstrate significantly inferior results. 3

  • Do not repeat antibiotics to which the patient has been previously exposed, especially clarithromycin and levofloxacin, as this maximizes treatment failure risk. 2

  • Inadequate PPI dosing is a major cause of treatment failure—always prescribe high-dose (twice daily) PPI, not standard once-daily dosing. 2, 4

  • Bismuth quadruple therapy is one of the least tolerable H. pylori regimens due to side effects, so counsel patients about potential gastrointestinal symptoms and the importance of adherence despite discomfort. 3

Special Populations

In patients with true penicillin allergy, amoxicillin can be replaced with tetracycline in alternative regimens, or use the standard bismuth quadruple therapy which does not contain penicillin. 1, 3

In children, avoid both fluoroquinolones and tetracyclines, which considerably limits treatment options. 2, 3

Verification of Eradication

Confirm eradication with urea breath test or monoclonal stool antigen test at least 4 weeks after completing therapy and at least 2 weeks after discontinuing PPIs. 2, 4 Never use serology to confirm eradication, as antibodies persist long after successful treatment. 2, 4

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Guideline

Helicobacter pylori Treatment Guidelines

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

Treatment of Helicobacter pylori Infections

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Guideline

H. pylori-Related Pain Treatment

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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