What is the recommended washout period for H1 antihistamines before performing skin prick or intradermal allergy testing?

Medical Advisory BoardAll articles are reviewed for accuracy by our Medical Advisory Board
Educational purpose only • Exercise caution as content is pending human review
Article Review Status
Submitted
Under Review
Approved

Last updated: February 9, 2026View editorial policy

Personalize

Help us tailor your experience

Which best describes you? Your choice helps us use language that's most understandable for you.

Antihistamine Washout Period Before Allergy Testing

Discontinue antihistamines for a minimum of 3-7 days before skin prick or intradermal allergy testing, with the specific duration depending on the antihistamine's elimination half-life.

Drug-Specific Washout Periods

The washout period must be tailored to the specific antihistamine being used, as elimination half-lives vary significantly:

  • Desloratadine requires 6 days due to its longest elimination half-life of 27 hours 1
  • Most second-generation antihistamines (cetirizine, fexofenadine, loratadine, levocetirizine) require 3-5 days based on their pharmacokinetic profiles 1
  • Ebastine requires 4 days, as research demonstrates the wheal and flare response returns to baseline by day 4 after discontinuation 2
  • First-generation antihistamines (hydroxyzine, chlorphenamine) require 3-5 days despite shorter half-lives, as they demonstrate more potent suppression of skin test responses 1

Critical Testing Considerations

Drugs with antihistamine activity must be discontinued several days before testing to avoid false-negative results 1. The guideline specifically states that "drugs with anti-histamine activity must be discontinued a few days before testing" 1.

Key points for accurate testing:

  • Antihistamines suppress both histamine and allergen-induced skin reactions, with first-generation agents like hydroxyzine showing >50% suppression of histamine responses even at standard doses 3
  • Testing can proceed as soon as the patient has made full clinical recovery from any acute allergic reaction and antihistamine effects have worn off 1
  • There is no need to discontinue oral or inhaled corticosteroids before skin testing 1

Alternative Testing Strategy

If antihistamines cannot be discontinued for medical reasons, proceed directly to serum-specific IgE blood testing instead of skin testing 4. The European Respiratory Society recommends this approach because:

  • Blood testing is not affected by antihistamine use 4
  • Sensitivity ranges from 0.58-0.90 and specificity from 0.56-0.65 compared to skin prick testing 4
  • This is particularly useful for patients with extensive eczema, dermographism, or urticaria where skin testing is impractical 4

Common Pitfalls to Avoid

Do not perform skin testing while patients are taking antihistamines, as this will result in false-negative results and missed diagnoses 1. The clinician should document all medications taken within the past week, as many medications beyond antihistamines (including tricyclic antidepressants) may suppress skin test responses 1.

Never use antihistamine washout as a reason to delay urgent testing—simply switch to blood-based IgE testing when immediate results are needed 4.

References

Guideline

Guideline Directed Topic Overview

Dr.Oracle Medical Advisory Board & Editors, 2025

Research

Suppression of histamine- and allergen-induced skin reactions: comparison of first- and second-generation antihistamines.

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, 2009

Guideline

Environmental Allergy Testing in Children

Praxis Medical Insights: Practical Summaries of Clinical Guidelines, 2025

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

Have a follow-up question?

Our Medical A.I. is used by practicing medical doctors at top research institutions around the world. Ask any follow up question and get world-class guideline-backed answers instantly.