How many milliliters of normal saline are needed to reconstitute a 36‑mg vial of retatrutide to achieve a concentration of 2 mg/mL?

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Last updated: February 25, 2026View editorial policy

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Reconstitution of Retatrutide 36mg to 2mg/mL

To achieve a concentration of 2 mg/mL from a 36 mg vial of retatrutide, you need to add 18 mL of normal saline.

Calculation Method

The calculation follows basic dilution principles where the desired concentration determines the final volume needed:

  • Target concentration: 2 mg/mL
  • Total drug amount: 36 mg
  • Required final volume: 36 mg ÷ 2 mg/mL = 18 mL
  • Volume of diluent to add: 18 mL of normal saline

Practical Considerations

  • Normal saline (0.9% sodium chloride) is the appropriate diluent for peptide medications, as it maintains isotonicity and stability without causing precipitation 1, 2

  • After adding 18 mL of normal saline to the 36 mg vial, gently swirl (do not shake vigorously) to ensure complete dissolution of the lyophilized powder

  • The reconstituted solution should be clear and free of particulate matter before administration

  • Label the vial clearly with the final concentration (2 mg/mL), date and time of reconstitution, and your initials

Storage and Stability

  • Once reconstituted, store the vial under refrigeration (2-8°C) and use within the timeframe specified by the manufacturer's guidelines

  • Protect from light and avoid freezing the reconstituted solution

  • Each dose can be drawn up using aseptic technique with an appropriate syringe for subcutaneous administration 3, 4, 5

Professional Medical Disclaimer

This information is intended for healthcare professionals. Any medical decision-making should rely on clinical judgment and independently verified information. The content provided herein does not replace professional discretion and should be considered supplementary to established clinical guidelines. Healthcare providers should verify all information against primary literature and current practice standards before application in patient care. Dr.Oracle assumes no liability for clinical decisions based on this content.

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